Можно использовать тег object, а на onmouseover и onmouseout выставить включение и отключение звука. Вот пример:
<object id="mediaPlayer" classid="CLSID:6BF52A52-394A-11D3-B153-00C04F79FAA6" type="application/x-oleobject" width="0" height="0"> <param name="URL" value="/files/misc/01-samba_cueca.mp3"> <param name="ShowControls" value="0"> <param name="AutoStart" value="true"> <param name="AutoRewind" value="true"> <param name="EnableContextMenu" value="false"> <param name="EnablePositionControls" value="true"> <param name="stretchToFit" value="false"> <param name="CurrentPosition" value="9"> <param name="PlayCount" value="100"> <param name="uiMode" value="none"> <embed src="/files/misc/01-samba_cueca.mp3" mce_src="/files/misc/01-samba_cueca.mp3" autostart="1" autorewind="1" currentposition="9" playcount="100" uimode="none" stretchtofit="0" type="application/x-mplayer2" width="0" border="0" height="0"></object>
Подробнее почитай в хелпниках..